Withanolides from Aureliana fasciculata var. fasciculata

In the first phytochemical study of the Aureliana genus (Solanaceae), two new withanolides, 1 and 2 , together with two known sterols, were isolated from the MeOH extract of the leaves of Aureliana fasciculata var. fasciculata. The structures were established as (4 S ,22 R )‐16 α ‐acetoxy‐5 β ,6 β ‐epoxy‐4 β ,17 α ‐dihydroxy‐1‐oxowitha‐2,24‐dienolide (aurelianolide A) and (4 S ,22 R )‐16 α ‐acetoxy‐4 β ,17 α ‐dihydroxy‐1‐oxowitha‐2,5,24‐trienolide (aurelianolide B). The new compounds possessed the unusual 16 α ,17 α ‐dioxygenated group and were fully characterized by spectroscopic techniques, including 1 H‐ and 13 C‐NMR (DEPT), as well as 2D‐NMR (HMBC, HMQC, 1 H, 1 H‐COSY, NOESY) experiments, and HR‐MS.