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Building‐Block Approach for the Straightforward Incorporation of a New FRET (Fluorescence‐Resonance‐Energy Transfer) System into Synthetic DNA
Author(s) -
Clima Lilia,
Bannwarth Willi
Publication year - 2008
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.200890008
Subject(s) - förster resonance energy transfer , chemistry , chromophore , acceptor , dna , fluorescence , combinatorial chemistry , energy transfer , photochemistry , chemical physics , biochemistry , physics , quantum mechanics , condensed matter physics
The synthesis of the two new phosphoramidites 5 and 8 bearing a carbostyril (=quinolin‐2(1 H )‐one) chromophore used as donor entity in our recently developed new FRET (fluorescence‐resonance‐energy transfer) system is described ( Schemes 1 and 2 ) The high stability of the chromophore to basic conditions enables the incorporation of the phosphoramidites directly into DNA during solid‐phase synthesis ( Schemes 3 and 4 ). Since this is also possible for the (bathophenanthroline)ruthenium(II) complex used as acceptor ( Scheme 4, Steps d and e ), the whole labelling procedure to insert the FRET system into synthetic DNA is straightforward and represents a major improvement to our previous strategy.