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Synthesis, DNA Binding, and DNA Photocleavage of the Ruthenium(II) Complexes [Ru(bpy)(btip)] 2+ and [Ru(dmb)(btip)] 2+ (bpy = 2,2′‐Bipyridine; btip = 2‐Benzo[ b ]thien‐2‐yl‐1 H ‐imidazo[4,5‐ f ] [1,10]phenanthroline; dmb = 4,4′‐Dimethyl‐2,2′‐bipyridine)
Author(s) -
Tan LiFeng,
Wang Fang,
Chao Hui
Publication year - 2007
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.200790017
Subject(s) - chemistry , ruthenium , intercalation (chemistry) , dna , bipyridine , ligand (biochemistry) , stereochemistry , singlet oxygen , radical , phenanthroline , medicinal chemistry , crystallography , catalysis , oxygen , organic chemistry , receptor , biochemistry , crystal structure
The new polypyridyl ligand btip (= 2‐benzo[ b ]thien‐2‐yl‐1 H ‐imidazo[4,5‐ f ] [1,10]phenanthroline) and its Ru II complexes [Ru(bpy) 2 (btip)] 2+ ( 1 ; bpy = 2,2′‐bipyridine) and [Ru(dmb) 2 (btip)] 2+ ( 2 ; dmb = 4,4′‐dimethyl‐2,2′‐bipyridine) were synthesized and characterized by elemental analysis, MS, and 1 H‐NMR. The DNA‐binding properties of the two complexes to calf‐thymus DNA (CT‐DNA) were investigated by different spectrophotometric methods and viscosity measurements. The results suggest that both complexes bind to CT‐DNA through intercalation. Also, when irradiated at 400 nm, the two complexes promote the photocleavage of plasmid pBR‐322 DNA. Thereby, under comparable experimental conditions, complex 1 cleaves DNA more effectively than complex 2 does. Mechanistic studies reveal that singlet oxygen ( 1 O 2 ) and hydroxyl radicals (OH . ) play a significant role in the photocleavage.