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Radio‐Analytical Determination of the Coating Efficiency of Cyclic RGD Peptides
Author(s) -
Auernheimer Jörg,
Haubner Roland,
Schottelius Margret,
Wester HansJürgen,
Kessler Horst
Publication year - 2006
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.200690085
Subject(s) - chemistry , adhesion , peptide , coating , cell adhesion , biophysics , cyclic peptide , tyrosine , methacrylate , cell growth , biochemistry , monomer , polymer , organic chemistry , biology
Abstract Coating of artificial surfaces with RGD (= arginine‐glycine‐aspartate) peptides to enhance cell adhesion is an ongoing issue. Thereby, the physiological adhesion process to the extra‐cellular matrix (ECM) is mimicked by the peptide coating, leading to a strong cell‐surface contact, followed by spreading and proliferation of the cells. For comparable cell adhesion studies, it is important to know the density of the RGD peptides on the surface. Here, we present an approach to determine the amount of bound cyclic RGD peptide by radio labeling with 125 I of a tyrosine‐containing RGD peptide on different materials surfaces (poly(methyl methacrylate) (PMMA), titanium, and silicon). For all surfaces, the amount of bound peptides is in the range of pmol/cm 1 .