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Synthesis and Triple‐Helix‐Stabilization Properties of Branched Oligonucleotides Carrying 8‐Aminoadenine Moieties
Author(s) -
Aviño Anna,
Grimau Marta G.,
Frieden Miriam,
Eritja Ramon
Publication year - 2004
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.200490028
Subject(s) - chemistry , triple helix , pyrimidine , oligonucleotide , stereochemistry , branching (polymer chemistry) , purine , molecule , dna , biochemistry , organic chemistry , enzyme
The synthesis of several branched oligonucleotides, i.e. , of the parallel hairpins 5 – 8 and the Y‐shaped 9 is described, together with their use in the formation of pyrimidine⋅pyrimidine⋅purine triple helices. Special attention was paid to the optimization of the assembly of the second strand from asymmetric branching molecules. The presence of 8‐aminoadenine moieties in the Watson Crick purine strand and 2′‐ O ‐methyl‐RNA in the Hoogsteen pyrimidine strand produced strong stabilization of the triplex.