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Synthesis of Branched Oligonucleotides as Templates for the Assembly of Nanomaterials
Author(s) -
Grimau Marta G.,
Iacopino Daniela,
Aviñó Anna,
de la Torre Beatriz G.,
Ongaro Andrea,
Fitzmaurice Donald,
Wessels Jurina,
Eritja Ramon
Publication year - 2003
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.200390232
Subject(s) - oligonucleotide , chemistry , nanomaterials , template , nanoparticle , conjugate , transmission electron microscopy , nanotechnology , combinatorial chemistry , dna , biochemistry , mathematical analysis , materials science , mathematics
Branched oligonucleotides with symmetric arms 6 – 15 , which may contain biotin as a second recognition code, were prepared. These molecules are designed to be used for the directed assembly of nanomaterials. The branched structure of the desired oligonucleotides was confirmed by mass spectrometry on small branched oliognucleotides, by gel electrophoresis, and by hybridization with complementary oligonucleotidenanoparticle conjugates, followed by visualization of the complexes by transmission electron microscopy.