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Evidence from Circular Dichroism and from Melting Behavior for Helix‐Inducing Complexation of a Designed β 3 ‐Pentadecapeptide with DNA Duplexes. Preliminary Communication
Author(s) -
Kimmerlin Thierry,
Namoto Kenji,
Seebach Dieter
Publication year - 2003
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.200390167
Subject(s) - circular dichroism , chemistry , dna , side chain , helix (gastropod) , crystallography , stereochemistry , melting temperature , biophysics , biochemistry , polymer , organic chemistry , ecology , materials science , snail , composite material , biology
Inspired by naturally occurring DNA‐binding proteins and their artificial α ‐peptidic mimics reported to date, a research project was initiated aiming at creating a new class of β ‐peptides capable of binding to and ultimately regulating the functions of DNA. As an initial foray, a β 3 ‐pentadecapeptide 1 , which bears H‐bonding Asn side chains and positively charged Lys side chains, was designed and synthesized on the solid support. DNA‐Complexation studies by means of circular dichroism and DNA‐melting‐temperature measurements revealed the first preliminary indications that support the existence of ordered interactions between β ‐peptides and DNA.