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Synthesis and Some Biochemical Properties of a Novel 5,6,7,8‐Tetrahydropyrimido[4,5‐ c ]pyridazine Nucleoside
Author(s) -
Loakes David,
Brown Daniel M.,
Salisbury Stephen A.,
McDougall Mark G.,
Neagu Constantin,
Nampalli Satyam,
Kumar Shiv
Publication year - 2003
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.200390103
Subject(s) - chemistry , klenow fragment , nucleoside , phosphoramidite , stereochemistry , base pair , exonuclease , primer (cosmetics) , polymerase , thymidine , dna , dna polymerase , nucleoside analogue , biochemistry , organic chemistry , oligonucleotide
A novel nucleoside analogue is described based on the pyridazine ring system. The nucleoside was successfully incorporated into DNA via both its phosphoramidite and 5′‐triphosphate derivatives. Enzymatically, the analogue behaves essentially as thymidine: it is a good substrate for the DNA polymerases Taq and exonuclease‐free Klenow fragment, leading to full‐length products when present in either the primer or template strands. In hybridisation studies, the nucleoside displays ambiguous base‐pairing properties, including universal base properties.