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Rapid Cleavage of Oligodeoxyribonucleotides from cis ‐Diol‐Bearing Universal Polymer Support
Author(s) -
Kumar Pradeep,
Gupta Kailash C.
Publication year - 2003
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.200390019
Subject(s) - chemistry , cleavage (geology) , spermine , nucleic acid , aqueous solution , diol , polymer , ion , combinatorial chemistry , stereochemistry , polymer chemistry , organic chemistry , biochemistry , enzyme , geotechnical engineering , fracture (geology) , engineering
Two sets of conditions have been proposed for the cleavage of oligodeoxyribonucleotides from cis ‐diol‐bearing polymer supports and their complete deprotection. The first condition involves the use of spermine in the presence of Zn 2+ ions in aqueous NH 3 at 60° for 1 h, while the second one employs the use of 0.5 M LiOH in conjunction with 3.5 M Et 3 N/MeOH at 75° for 40–60 min, or under microwaves for 5–8 min, depending on the protection employed for nucleic bases.