Premium
A Study of the Metabolic Degradation of an insect juvenile hormone analog using different radiolabeling
Author(s) -
Tykva Richard,
Wimmer Zdenêk,
Bennettová Blanka,
Vlasáková Vêra
Publication year - 1998
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19980810115
Subject(s) - chemistry , juvenile hormone , insect , flesh fly , degradation (telecommunications) , hormone , biochemistry , larva , botany , telecommunications , biology , computer science
The fate of an insect juvenile hormone analog applied to the insect body of the flesh fly ( Sarcophaga bullata ) or the tsetse fly ( Glossina palpalis ), respectively, was studied using three different radiolabeled positions in the parent biologically active compound 1 . Several metabolites were found and analyzed. A mechanism of degradation of the applied molecule was designed using a combination of several radioanalytical methods. A unique monitoring of the applied compound and its metabolites was provided, based on the different radiolabeling of the structure ( cf. 1a – 1c ), and established their fate in an insect body during a 10‐day experiment. A 14 C and 3 H radiolabeling, respectively, was employed to synthesize three different radiolabeled forms 1a – 1c derived from the parent non‐labeled 1 . A combination of three different ways of radiolabeling resulted in an advantage in tracing the metabolic pathway of degradation of the employed compound 1 in its radiolabeled forms 1a – 1c .