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Molecular Recognition of NADP(H) and ATP by Macrocyclic Polyamines Bearing Acridine Groups
Author(s) -
Fenniri Hicham,
Hosseini Mir Wais,
Lehn JeanMarie
Publication year - 1997
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19970800314
Subject(s) - chemistry , acridine , polyamine , nucleic acid , nad+ kinase , stereochemistry , stacking , fluorescence , nucleotide , biochemistry , selectivity , in vitro , molecular recognition , enzyme , molecule , organic chemistry , gene , physics , quantum mechanics , catalysis
The macrocyclic polyamine‐based receptor BA bearing two acridine units makes use of combined electrostatic and stacking interactions for the binding of nucleotide polyphosphates and for the recognition of ATP and of NADPH ( k a > 3 · 10 8 M), with a high selectivity for NADPH vs. NADP ( ca. 10 3 ) and NAD(H) (> 10 6 ). The binding properties of this receptor towards a variety of substrates led to its in vitro application as a fluorescent probe for ATP. BA also interacts strongly with nucleic acids as shown by spectrophotometric, spectrofluorimetic, and electrophoretic mobility methods.