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Development of Luminescent Terbium(III) Chelates for Protein Labelling: Effect of triplet‐state energy level
Author(s) -
Takalo Harri,
Mukkala VeliMatti,
Meriö Liisa,
RodríguezUbis Juan Carlos,
Sedano Rosa,
Juanes Olga,
Brunet Ernesto
Publication year - 1997
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19970800204
Subject(s) - chemistry , terbium , labelling , luminescence , chelation , triplet state , photochemistry , inorganic chemistry , organic chemistry , biochemistry , molecule , optoelectronics , ion , physics
The synthesis of novel Tb III labels suitable for protein labelling are reported. Their luminescence properties as antibody conjugates were measured and compared to the results of corresponding Tb III chelates of the parent ligand structures. When the lowest triplet‐state energy level of the parent donor ligand was over 23000 cm −1 , i.e. , the energy gap between the 5 D 4 level of Tb III and the lowest triplet‐state energy level of the ligand exceeded 2600 cm −1 , the label derivative with a long decay time (τ = 1.35–2.93 ms) and a high luminescence yield (ϵ. Φ = 3770–4560) was found to be suitable for bioaffinity assays.