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Interaction of Double‐Helical Polynuclear Copper(I) complexes with double‐stranded DNA
Author(s) -
Schoentjes Bruno,
Lehn JeanMarie
Publication year - 1995
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19950780103
Subject(s) - chemistry , dna , ecorv , cleavage (geology) , pbr322 , nucleic acid , stereochemistry , crystallography , double stranded , helix (gastropod) , copper , restriction enzyme , plasmid , ecori , biochemistry , organic chemistry , ecology , geotechnical engineering , fracture (geology) , snail , biology , engineering
Double‐helical metal complexes, the helicates H 2 –H 5 , were found to bind to double‐helical DNA by spectroscopic, DNA‐melting, and electrophoretic‐mobility measurements. The helicates also inhibited the cleavage of DNA by two restriction enzymes, Sspl and EcoRV, a property which agrees with the binding occurring in the major groove of the double helix. Visible‐light irradiation of solutions containing a helicate and the pBR322 plasmid led to single‐strand cleavage, indicating that these complexes could be of interest as potential probes for nucleic‐acid structure.