Premium
Derivatisierung von PQQ und PQQ‐Triester für den Nachweis von PQQ
Author(s) -
Martin Pierre,
Winkler Tammo
Publication year - 1993
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19930760425
Subject(s) - chemistry , pyrroloquinoline quinone , adduct , pyrrole , indole test , cofactor , organic chemistry , chromatography , enzyme
Formation of derivatives of PQQ and PQQ‐Triester for the Detection of PQQ PQQ is an organic redox/cofactor found in a class of enzymes called quinoproteins. It has been reported that materials like milk, egg yolk, vinegar, beer, serum, urine, and biological tissues contain little amounts of PQQ . To facilitate studies on PQQ , a simple detection of PQQ could be extremely useful. For this purpose, we describe in this paper some adducts of PQQ or PQQ ‐triester with 1,2‐diamines, 2,4‐dihydroxyaniline, pyrrole, indole as well as an oxidation product of PQQ . UV and fluorimetric detections of these colored products show that this method is relatively sensitive. The reaction of PQQ ‐triester with (MeO) 3 P to the phosphole adduct 13 is of great importance: the use of [ 32 P]‐labeled trialkyl phosphites will make this reaction an extremely sensitive method to detect PQQ .