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Assignment of 15 N‐NMR resonances of vitamin B 12 analogues by 2D‐[ 15 N, 1 H] long‐range correlation: Fully [ 15 N]‐labelled Co‐β‐cyano‐5‐hydroxybenzimidazoylcobamide (factor III) and derivatives
Author(s) -
Hollenstein† Roger,
Stupperich Erhard
Publication year - 1993
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19930760312
Subject(s) - chemistry , ligand (biochemistry) , resonance (particle physics) , nuclear magnetic resonance spectroscopy , nmr spectra database , spectroscopy , crystallography , spectral line , stereochemistry , analytical chemistry (journal) , atomic physics , physics , biochemistry , receptor , quantum mechanics , astronomy , chromatography
The 15 N‐NMR spectra of vitamin B 12 analogues obtained in fully 15 N‐labelled form have been measured by direct and inverse ( 15 N, 1 H) correlated spectroscopy. All resonances, except those of the NH 2 groups, have been assigned to individual N‐atoms. The influences on δ (N) are analyzed and discussed which are caused by changing the α‐face ligand from CN to H 2 O or CH 3 and by switching the β‐face ligand from the base‐on to the base‐off state. An implication of the correct resonance assignment on biosynthetic pathways is demonstrated.