Premium
Warum pentose‐und nicht hexose‐nucleinsäuren? Teil III . Oligo(2′,3′‐dideoxy‐β‐ D ‐glucopyranosyl) nucleotide (‘homo‐DNS’): Paarungesigenschaften
Author(s) -
Hunziker Jürg,
Roth HansJorg,
Böhringer Markus,
Giger Alfred,
Diederichsen Ulf,
Göbel Michael,
Krishnan Ravichandran,
Jaun Bernhard,
Leumann Christian,
Eschenmoser Albert
Publication year - 1993
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19930760119
Subject(s) - chemistry , oligonucleotide , nucleotide , pentose , hexose , dna , pairing , nucleic acid , nuclear magnetic resonance spectroscopy , stereochemistry , base pair , biochemistry , crystallography , gene , enzyme , physics , superconductivity , quantum mechanics , fermentation
Why Pentose‐And Not Hexose‐Nucleic Acids? Part III. Oligo(2′,3′‐dideoxy‐β‐ D ‐glucopyranosyl)nucleotides. (‘Homo‐DNA’): Base‐Pairing PropertiesSummary in collaboration with Prof. Dr. C. E. Wintner , Haverford College, Haverford, PA 19041‐1392. The paper presents results of a comprehensive investigation on the pairing properties of homo‐DNA oligonucleotides, the preparation of which has been described in Part II of this series [2]. The investigation was carried out by using established methods described in the literature for the characterization of oligonucleotides in the natural series, such as determination of melting temperatures of oligonucleotide duplexes by temperature‐dependent of melting temperatures, determination of pairing stoichiometry by ratio‐dependent UV spectroscopy of binary mixtures of pairing partners, temperature‐dependent CD spectroscopy, gel electrophoresis under non‐denaturing conditions, and – in selected cases – 1 H – and 31 P‐NMR spectroscopy. The systematic comparison of the paring properties of homo‐DNA oligonucleotides with corresponding DNA nucleotides (up to dodecamers) indicates that homo‐DNA is a highly efficient, autonomous, artificial pairing system with a pairing behavior that is in part similar to, but also, in part, strikingly different from, the pairing behavior of DNA. The pairing properties established so far are listed below in a manner that reflects the sequence of subtitles in Chapt.2 of the text; they were determined under the conditions: H 2 O, 0.15 M NaCl, 0.01 M Tris‐HCl buffer, pH 7, oligonucleotide concentrations in the μ M range, 1:1 ratio of single strands in the case of non‐selfcompementary sequences.