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Nucleotides. Part XXXVIII. . Syntheses and characterization of phosphorothioate analogues of (2′–5′) adenylate dimer and trimer and their 5′‐ O ‐monophosphates
Author(s) -
Charubala Ramamurthy,
Pfleiderer Wolfgang
Publication year - 1992
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19920750206
Subject(s) - chemistry , trimer , dimer , diastereomer , adenylate kinase , stereochemistry , phosphoramidite , nucleotide , organic chemistry , biochemistry , oligonucleotide , dna , gene , enzyme
The chemical syntheses of the phosphorothioate of (2′–5′)adenylate dimer (see 6a , 6b ) and trimer (see 11a , 11b , 12a , 12b ) as well as of their 5′‐monophosphates (see 15a , 15b , 16a , 16b ) using the phosphoramidite method are described. The resulting diastereoisomer mixtures were separated into the pure components by chromatographical means. All synthetic intermediates were characterized by TLC, elemental analysis, and UV and 1 H‐NMR spectra.