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Energy Transfer from a Lumazine (= Pteridine‐2,4(1 H ,3 H )‐dione) Chromophore to Bathophcnanthroline‐ruthenium(II) Complexes during Hybridization Processes of DNA
Author(s) -
Bamwarth Willi,
Müller Francis
Publication year - 1991
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19910740835
Subject(s) - chemistry , chromophore , oligonucleotide , ruthenium , pteridine , dna , stereochemistry , energy transfer , combinatorial chemistry , acceptor , photochemistry , organic chemistry , biochemistry , catalysis , chemical physics , physics , enzyme , condensed matter physics
We incorporated 6,7‐dimethyllumazine chromophores and a [Ru II (bathophenanthroline)] complex into different oligodeoxynucleotides, the former via the corresponding phosphoramidites of the 2′‐deoxynucleosides and the latter specifically by the phosphonate approach at the 5′‐end of synthetic oligonucleotides, directly in the course of their synthesis on solid support. Efficiency measurements of this energy‐transfer system with the lumazine as energy donor and the Ru complex as energy acceptor allowed us to distinguish between the hybridized and the non‐hybridized state of oligonucleotides. Thus, the approach should be of value in DNA probe technology for the detection of single‐stranded DNA targets.