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A New Combined Purification/Phosphorylation Procedure for Oligodeoxynucleotides
Author(s) -
Banmvarth Willi,
Wippier Jürgen
Publication year - 1990
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19900730434
Subject(s) - chemistry , sequence (biology) , combinatorial chemistry , covalent bond , dna , phosphorylation , oligonucleotide , matrix (chemical analysis) , chromatography , computational biology , biochemistry , organic chemistry , biology
We describe a new principle for the purification of oligodeoxynucleotides using a purification handle which leads to a covalent linkage of the desired DNA sequence to a solid matrix. Truncated sequences are removed by washing steps. The release of the pure target sequence is performed in such a way that it is obtained directly in its 5′‐phosphorylated form which represents a further advantage. In addition, the system can be used for 5′‐thiol modifications of synthetic DNA.