Premium
Nucleotides part XXX Chemical synthesis of adenylyl‐(2′ → 5′)‐adenylyl‐(2′ → 5′)‐8‐azidoadenosine, and activation and photoaffinity labelling of RNase L by [ 32 P]p5′A2′p5′A2′p5′N 3 8 A
Author(s) -
Charubala Ramamurthy,
Pfleiderer Wolfgang,
Sobol Robert W.,
Wu Li Shi,
Suhadolnik Robert J.
Publication year - 1989
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19890720624
Subject(s) - chemistry , labelling , rnase p , adenylyl cyclase , photoaffinity labeling , stereochemistry , covalent bond , affinity label , enzyme , nucleotide , biochemistry , binding site , rna , organic chemistry , gene
The chemical synthesis of adenylyl‐(2′–5′)‐adenylyl‐(2′–5′)‐8‐azidoadenosine ( 15 ) was performed by the phosphotriester approach. Enzymatic phosphorylation of 15 by [γ‐ 32 P]ATP led to the corresponding labelled 5′‐monophosphate 16 . Photoinsertion of 16 took place on UV irradiation by covalent cross linking to a protein of M r 80 K known to be RNase L. Radiobinding and core‐cellulose assays as well as photoaffinity labelling experiments with 16 are described.