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Template‐Dependent Incorporation of Spin‐Labeled Thymidine Analogs into Viral DNA
Author(s) -
Pauly Gary T.,
Bobst Elisabeth V.,
Bruckman David,
Bobst Albert M.
Publication year - 1989
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19890720114
Subject(s) - chemistry , dna polymerase , thymidine , dna , polymerase , radical , nitroxide mediated radical polymerization , dna synthesis , stereochemistry , combinatorial chemistry , microbiology and biotechnology , biochemistry , organic chemistry , radical polymerization , copolymer , biology , polymer
The synthesis of novel pppU d analogs substituted at position C(5) with tethered nitroxide radicals is reported. It is shown that these analogs can serve as good substrates for E. coli DNA polymerase (Pol I) and T‐4 DNA polymerase using lambda‐phage DNA as template. The template‐dependent incorporation of the substrates was established by radio‐labeling and ESR experiments.