Premium
Binding of AMP, ADP, and ATP Nucleotides by Polyammonium Macrocycles
Author(s) -
Hosseini Mir Wais,
Lehn JeanMarie
Publication year - 1987
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19870700512
Subject(s) - chemistry , protonation , pyrophosphate , nucleotide , titration , stoichiometry , aqueous solution , stereochemistry , adenine nucleotide , nuclear magnetic resonance spectroscopy , molecule , adenosine diphosphate , inorganic chemistry , enzyme , biochemistry , organic chemistry , ion , platelet , platelet aggregation , biology , immunology , gene
The macrocyclic polyamines 4 – 6 , when protonated, bind strongly and selectively nucleotides (AMP, ADP, ATP) and pyrophosphate in aqueous solution. The stoichiometry of the complexes formed was determined by titration experiments followed by 31 P‐NMR spectroscopy. Compounds 4 and 5 form 1:1 complexes with ATP, ADP, and pyrophosphate, whereas 6 forms complexes with ATP and ADP involving 2 nucleotides and 1 receptor molecule. The stability constants of these complexes have been determined by pH‐metric measurements. At pH 7, both 5 and 6 give complexes of mainly the fully protonated species 5 . 6H + and 6 . 8H + , whereas 4 yields predominantly complexes of 4 . 5H + and 4 . 4H + .