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Nucleotides. Part XXVII Bis[2‐( p ‐nitrophenyl)ethyl] Phosphorochloridate, a New Versatile Phosphorylating Agent in Nucleotide Chemistry
Author(s) -
Himmelsbach Frank,
Charubala Ramamurthy,
Pfleiderer Wolfgang
Publication year - 1987
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19870700508
Subject(s) - chemistry , synthon , nucleotide , oligonucleotide , phosphorylation , stereochemistry , combinatorial chemistry , organic chemistry , biochemistry , dna , gene
A new, versatile phosphorylating agent, bis[2‐( p ‐nitrophenyl)ethyl] phosphorochloridate ( 3 ), has been prepared and is used for 3′‐ and/or 5′‐phosphorylations of nucleosides. The resulting bis[2‐( p ‐nitrophenyl)ethyl] phosphotriesters are versatile synthons in oligonucleotide synthesis leading finally to 3′‐ and/or 5′‐terminated monophosphates in excellent yields.