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Three Novel Spin‐Labeled Substrates for Enzymatic Incorporation into Nucleic Acid Lattices
Author(s) -
Toppin Charles R.,
Pauly Gary T.,
Devanesan Prabu,
Kryak David,
Bobst Albert M.
Publication year - 1986
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19860690213
Subject(s) - chemistry , substrate (aquarium) , dna polymerase , polymerase , deoxyuridine , uridine , stereochemistry , polynucleotide , nitroxide mediated radical polymerization , nucleic acid , dna , enzyme , biochemistry , rna , organic chemistry , copolymer , oceanography , radical polymerization , gene , geology , polymer
The synthesis of one C(4)‐spin‐labeled uridine‐5′‐diphosphate (C(4)‐UDP) and two nitroxide‐containing 2′‐deoxyuridine‐5′‐triphosphate (dUTP) analogs are reported. The C(4)‐UDP derivative was incorporated into copolymers by polynucleotide phosphoxylase (PNPase); one of the two dUTP analogs, substituted at position C(4), was a good substrate for TdT, whereas the other one, substituted at position C(5), served as substrate for E. coli DNA polymerase (Pol I).