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Preparative Asymmetric Reduction of 3‐Ketobutyrate and ‐valerate by Suspended Cells of Thermophilic Bacteria ( Thermoanaerobium brockii ) in Ordinary Laboratory Equipment
Author(s) -
Seebach Dieter,
Giovannini Fabio,
Lamatsch Bernd
Publication year - 1985
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19850680420
Subject(s) - chemistry , thermophile , bacteria , microorganism , yield (engineering) , yeast , bioreactor , centrifugation , chromatography , biochemistry , organic chemistry , enzyme , genetics , materials science , metallurgy , biology
The thermophilic and anaerobic bacteria specified in the title are isolated on a 0.8 kg scale by tangential flow filtration and centrifugation from a 300‐1 bioreactor. The microorganisms are stored in a freezer (−20°) and used, analogously to baker's yeast, for asymmetric reductions. Thus, ethyl 3‐ketovalerate (4.3) g/1 (H 2 O) is converted in 40% yield to ( S )‐3‐hydroxyvalerate ( 6 ), with an enantiomeric excess of 93% (24 h at 72°).