Premium
Disproof of the Overall Enzymatic Biosynthesis of Vindoline from Tryptamine and Secologanin by Cell‐Free Extracts from the Leaves of Catharanthus roseus (L.) G. D ON
Author(s) -
Stöckigt Joachim,
Gundlach Heidrun,
DeusNeumann Brigitte
Publication year - 1985
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19850680204
Subject(s) - vindoline , catharanthus roseus , tryptamine , chemistry , alkaloid , indole alkaloid , biosynthesis , biochemistry , enzyme , stereochemistry , chromatography , vinblastine , biology , genetics , chemotherapy
A cell‐free extract, which was isolated from the leaves of mature Catharanthus roseus plants by a previously published procedure, does not convert a mixture of secologanin and radiolabelled tryptamine to vindoline, as was recently claimed. The radioactivity in the purified alkaloid extract determined by earlier workers is certainly due to ‘impurities’ in the presumed ‘vindoline’. This was shown by extensive purification of the alkaloid extract (which contained added unlabelled vindoline as a carrier) followed by chemical conversion of vindoline to two derivatives and subsequent purification, ultimately giving unlabelled deacetylvindoline.