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Improved Synthesis of Oligodeoxyribonucleotides by Solid‐Phase Phosphotriester Method Utilizing O 6 ‐[2‐( p ‐Nitrophenyl)ethyl]‐2′‐deoxyguanosine Derivatives
Author(s) -
Chollet André,
Ayala Edgar,
Kawashima Eric H.
Publication year - 1984
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19840670521
Subject(s) - chemistry , deoxyguanosine , combinatorial chemistry , solid phase synthesis , polystyrene , dna , nucleic acid , phase (matter) , stereochemistry , organic chemistry , biochemistry , polymer , peptide
The synthesis of oligodeoxyribonucleotides on a cross‐linked polystyrene solid support utilizing stable mono‐ and dinucleotide phosphotriester building blocks is presented. The use of O 6 [2‐( p ‐nitrophenyl)ethyl]‐2′‐deoxyguanosine derivatives yields cleaner DNA fragments by suppressing side reactions. Modifications improving the phosphotriester methodology are presented. The purification methods and analysis of synthetic oligodeoxyribonucleotides are described.