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Immobilized Enzyme Particles Prepared by Radiation Polymerization of Polyurethane Prepolymer
Author(s) -
Kumakura Minoru,
Kaetsu Isao
Publication year - 1983
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19830660846
Subject(s) - prepolymer , polyurethane , chemistry , polymerization , polymer chemistry , monomer , particle (ecology) , dispersion polymerization , immobilized enzyme , methacrylate , chemical engineering , enzyme , polymer , organic chemistry , oceanography , engineering , geology
The immobilization of enzymes such as cellulase by radiation polymerization of dispersed polyurethane prepolymer was studied using tolylene‐2,4‐diisocyanate and 2‐hydroxyethyl methacrylate. The polyurethane particles were obtained by the dispersion of polyurethane prepolymer followed by radiation polymerization, in which the enzyme was immobilized on its surface by convalent bonding. The particle diameter of immobilized enzyme particles varied with monomer concentration and composition. The enzymatic activity of immobilized enzyme particles varied with the temperature of dispersion and irradiation, and decreased with increasing particle diameter.