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Correction for Inner Filter Effects in Fluorescence Spectroscopy
Author(s) -
Lutz HansPeter,
Luisi Pier Luigi
Publication year - 1983
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19830660704
Subject(s) - chemistry , fluorescence , absorption (acoustics) , intensity (physics) , radiation , fluorescence spectroscopy , filter (signal processing) , spectroscopy , absorption spectroscopy , radiant intensity , diagonal , optics , analytical chemistry (journal) , physics , computer science , geometry , mathematics , chromatography , quantum mechanics , computer vision
A method is proposed for correcting experimental fluorescence readings for inner filter effects, i.e. the absorption of the exciting light and/or the absorption of the emitted radiation, which cause the non‐linearity between fluorescence intensity and fluorophor concentration. Basically the method consists of measuring the fluorescence intensity at two different points along the diagonal in the cell. Unlike similar methods proposed in the literature, the two points are corrected simultaneously for both absorption of excitation and of emission radiation without the necessity of reading the optical density of the solution, and with a very simple data elaboration.