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Simplified Liquid‐Phase Preparation of Four Decadeoxyribonucleotides and their Preliminary Spectroscopic Characterization
Author(s) -
Denny William A.,
Leupin Werner,
Kearns David R.
Publication year - 1982
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19820650803
Subject(s) - random hexamer , chemistry , tetramer , monomer , dimer , trimer , nuclear magnetic resonance spectroscopy , crystallography , stereochemistry , chromatography , organic chemistry , polymer , enzyme
Four self‐complementary decadeoxyribonucleotides, dApTpApTpApTpApTpApT, dApTpApTpCpGpApTpApT, dApTpApTpGpCpApTpApT and dApApApApApTpTpTpTpT, were chemically synthesized by the phosphotriester procedure. Efforts were concentrated on keeping the procedure as simple as possible, concomitant with obtaining high‐purity products at each step of the process. The decamers were elaborated from the 3'‐end, starting with a 3'− O ‐benzoylated monomer according to the scheme: monomer + monomer → dimer + dimer → tetramer + dimmer → hexamer + tetramer → decamer. Purity of intermediates and of the fully blocked decamers were monitored by chromatography and by 300‐MHz 1 HN M R. spectroscopy. The deblocked decadeoxyribonucleotides were characterized by their UV., CD., and 1 HNMR. spectra.