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Biosynthesis of the Indole Alkaloids. Cell‐free Systems from Catharanthus roseus Plants
Author(s) -
Kutney James P.,
Choi Lewis S. L.,
Honda Toshio,
Lewis Norman G.,
Sato Toshitsugu,
Stuart Kenneth L.,
Worth Brian R.
Publication year - 1982
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19820650716
Subject(s) - vindoline , catharanthus roseus , tryptamine , chemistry , biosynthesis , indole alkaloid , indole test , alkaloid , apocynaceae , stereochemistry , vinblastine , enzyme , biochemistry , botany , biology , genetics , chemotherapy
Cell‐free systems from Catharanthus roseus plants are utilized for various studies relating to the biosynthesis of indole alkaloids. Tryptamine ( 5 ) and secologanin ( 6 ), two fundamental building units, are shown to be incorporated into the alkaloid vindoline ( 7 ). In another study, catharanthine ( 18 ) and vindoline ( 7 ) are utilized by this enzyme system and coupled to the important bisindole biointermediate 3′,4′‐anhydrovinblastine The previously [20] used name for 17 , 3′, 4′‐dehydrovinblastine, is incorrect. ( 17 ). The latter substance is, in turn, incorporated and converted to the natural alkaloids leurosine ( 8 ), Catharine ( 9 ) and vinblastine ( 10 ), thereby providing information about the biosynthesis of these complex molecules. High pressure liquid chromatography assay of the enzymic mixture sheds light on the enzymes involved in the coupling of 18 and 7 .