Premium
Alkaloid Production in Catharanthus Roseus Cell Cultures. XI . Biotransformation of 3′, 4′‐Anhydrovinblastine to Other Bisindole Alkaloids
Author(s) -
Kutney James P.,
Aweryn Barbara,
Choi Lewis S. L.,
Kolodziejczyk Pawel,
Kurz Wolfgang G. W.,
Chatson Kenneth B.,
Constabel Friedrich
Publication year - 1982
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19820650417
Subject(s) - catharanthus roseus , biotransformation , chemistry , alkaloid , apocynaceae , suspension culture , stereochemistry , cell culture , yield (engineering) , organic chemistry , botany , biochemistry , enzyme , biology , genetics , materials science , metallurgy
Biotransformation studies of 3′,4′‐anhydrovinblastine (1) in Calharanthus roseus cell suspension cultures are reported. A detailed study of one cell line, coded at „916”, has revealed that 1 is transformed to the natural bisindole alkaloids leurosine ( 2 ) and Catharine ( 3 ) in approximately 25–30 and 9–16% yield, respectively. In 24–48 h, about 40% of 1 is utilized by the cells. Conditions for biotransformation of 1 have not been optimized so the above yields quoted are not final.