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Alkaloid Production in Catharanthus roseus Cell Cultures. IV . Characterization of the 953 Cell Line
Author(s) -
Kurz Wolfgang G. W.,
Chatson Kenneth B.,
Constabel Friedrich,
Kutney James P.,
Choi Lewis S. L.,
Kolodziejczyk Pawel,
Sleigh Stephen K.,
Stuart Kenneth L.,
Worth Brian R.
Publication year - 1980
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19800630714
Subject(s) - catharanthus roseus , chemistry , alkaloid , indole alkaloid , cell culture , apocynaceae , yohimbine , fermentation , botany , stereochemistry , biochemistry , biology , genetics , receptor , antagonist
The 953 cell line, one of the cell lines from Catharanthus roseus , has been investigated in terms of its ability to produce indole alkaloids. Various parameters (mitotic index, pH, cell dry weight, growth period) were evaluated in the fermentation process and their relative importance for alkaloid production was determined. The alkaloids isolated and identified are ajmalicine, yohimbine, isositsirikine, vallesiachotamine, strictosidine lactam, lochnericine, hörhammericine, hörhammerinine, vindolinine, 19‐epivindolinine, 19‐acetoxy‐11‐methoxytabersonine, 19‐hydroxy‐11‐methoxytabersonine and dimethyltryptamine.