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Specific Ligands for the Affinity Chromatography of Cholinergic Proteins
Author(s) -
Riggio Gaetano,
Hopff Wolfgang Herbert,
Hofmann Alfred André,
Waser Peter Gaudenz
Publication year - 1980
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19800630221
Subject(s) - chemistry , affinity chromatography , ligand (biochemistry) , cholinergic , hydrolysis , covalent bond , chromatography , acetylcholine receptor , cholinesterase , biochemistry , enzyme , organic chemistry , receptor , medicine , neuroscience , biology
Affinity chromatography of proteins requires a ligand covalently bound to a solid support separated by a spacer of sufficient length. In the specific case of acetyl‐cholinesterase we have reduced the conventional spacer synthesis from five to three steps. For affinity chromatography of cholinergic proteins the ideal ligand would be acetylcholine which, however, could not be used because it is easily hydrolyzed. We synthesized hydrolysis‐resistent ligands. Different specific ligands were synthesized for the affinity chromatography of serum esterase.