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The fluorescence of biliverdin dimethyl ester
Author(s) -
Braslavsky Silvia E.,
Holzwarth Alfred R.,
Lehner Harald,
Schaffner Kurt
Publication year - 1978
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19780610623
Subject(s) - chemistry , fluorescence , biliverdin , quantum yield , yield (engineering) , base (topology) , photochemistry , excitation , analytical chemistry (journal) , organic chemistry , heme , optics , mathematical analysis , physics , heme oxygenase , materials science , mathematics , metallurgy , enzyme , electrical engineering , engineering
Freshly prepared solutions of biliverdin dimethyl ester ( 2 ) in ethanol showed fluorescence maxima at 710 and 770 nm [Φ F = 1.1. 10 −4 (room temperature) and 5.0 10 −4 (77 K)]. The maxima of monoprotonated 2 at 77 K were shifted to 725 and 806 nm and the quantum yield was increased to 2.6. 10 −2 . This acid effect was reversible by neutralization with base. When a neutral solution was kept standing in the dark at room temperature, or when an acidic solution was neutralized by base, an additional fluorescence maximum at 500 nm with a mirror image excitation spectrum with λ max = 470 nm developed, which disappeared on addition of acid and which is attributed to a chemical change of 2 .