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Antigen Synthesis: The Preparation of Selected Dodecapeptide Carriers with Systematically Altered Structures by a Two‐Phase Method
Author(s) -
Schneider C. H.,
Wirz W.
Publication year - 1972
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19720550403
Subject(s) - chemistry , antigenicity , reagent , peptide , lysine , antigen , conjugate , combinatorial chemistry , peptide synthesis , glycine , phase (matter) , solid phase synthesis , epitope , biochemistry , amino acid , organic chemistry , mathematical analysis , genetics , mathematics , biology
The synthesis of a series of dodecapeptide ethyl esters containing L ‐leucine, L ‐ala‐nine, glycine and L ‐lysine is described. These peptides are suitable carriers for the preparation of haptenic conjugates to be used in antigenicity studies. The strategy for preparing intermediary hexapeptides has been called a two‐phase approach since the elongation of the peptide chain is carried out stepwise in a water‐immiscible organic phase, whereas removal of side‐products and excess reagent is effected by means of a suitably adapted aqueous phase. The method allows an efficient production of relatively large amounts of fully defined peptide antigens.