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Hormon‐Rezeptor‐Beziehungen: Synthese und eigenschaften von N ε ‐dansyllysin 21 ‐adrenocorticotropin‐(1–24)‐tetrakosipepid
Author(s) -
Schwyzer R.,
Schiller P. W.
Publication year - 1971
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19710540313
Subject(s) - chemistry , intramolecular force , receptor , stereochemistry , fluorescence , moiety , peptide hormone , substituent , hormone , biochemistry , physics , quantum mechanics
We are investigating interactions between hormones and their potential receptor molecules by means of biologically active, synthetic hormone derivatives. The substituents are so chosen that they can supply quantitative information about specific contacts or convert the hormone to an ‘affinity marker’. We describe the synthesis of N ε ‐dansyllysine 21 ‐adrenocorticotropin‐(1–24)‐tetrakosipeptide. In fat cells and in adrenal cells of the rat the dansyl substituent does not seem to impair the interaction between the peptide moiety and its biological receptors. It allows for affinity studies by fluorescence depolarisation and for measurement of intramolecular and intermolecular distances by means of energy transfer (fluorescence sensibilisation).