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Fluoreszenzmethoden zur histochemischen Sichtbarmachung von Monoaminen. 6 . Identifizierung der fluoreszierenden Produkte aus m ‐Hydroxyphenyläthylaminen und Formaldehyd
Author(s) -
Corrodi H.,
Jonsson G.
Publication year - 1966
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19660490123
Subject(s) - chemistry , formaldehyde , fluorescence , fluorescence microscope , nuclear chemistry , tyramine , chromatography , organic chemistry , biochemistry , physics , quantum mechanics
It is known that in tissue slices treated with dry gaseous formaldehyde according to the histochemical technique of H ILLARP & F ALCK structures containing catecholamines show a green fluorescence in the fluorescence microscope. The m ‐hydroxyphenylethylamines m ‐tyramine, m ‐hydroxyamphetamine and m ‐hydroxynorephedrine (metaraminol) enclosed in vitro in dry protein films undergo a similar reaction with dry gaseous formaldehyde and form fluorescent products which have been characterized as 6‐hydroxy‐3,4‐dihydro‐isoquinolines existing predominantly in the quinoidal form fluorescing at 385/510 nm. By treatment of these protein layers with gaseous hydrogen chloride another form fluorescing at 360/420 nm can be obtained which is not visible in the fluorescence microscope with the usual equipment.