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Eine partikelgebundene Transfer‐Ribonucleinsäure aus Rattenleber
Author(s) -
Hicklin A.,
Leuthardt F.
Publication year - 1963
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19630460407
Subject(s) - chemistry , ribonuclease , incubation , enzyme , biochemistry , microsome , nucleic acid , sephadex , ribosome , chromatography , amino acid , transfer rna , rna , gene
Incubation of microsonics with the supernatant of pH−5‐enzymes is as active in incorporating amino‐acids into proteins (controlled by use of leucin‐[ 14 C]) as incubation with the usual system, which is supplied with pH−5‐enzymes. Since the supernatant of pH−5‐enzymes does not contain any soluble ribonucleic acid (sRNA), all the le∼ciri‐[ 14 C] found in nucleic acids must be hound to microsonial KNA. The behaviour of the isolated microsomal KNA on Sephadex G 75, and of the ribonuclease digest in paper electrophoresis, confirms our suggestion of the presence of an active sRNA fraction in microsomes and ribosomes.