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Ein Verfahren zur quantitativen Isolierung reiner Sphingomyeline aus Menschen‐ und Rattenhirn
Author(s) -
Hausheer L.,
Pedersen W.,
Bernhard Karl
Publication year - 1963
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19630460220
Subject(s) - chemistry , sodium methoxide , chromatography , yield (engineering) , organic chemistry , methanol , materials science , metallurgy
Abstract A chromatographic method for the isolation of sphingomyelines from human and rat brain is reported. After isolation of the total lipids we first separate lecithine and sphingomyeline, on a silicagel column. Afterwards lecithine is split by transesterification with sodium methoxide. The cleavage products are separated from sphingomyeline on a silicagel column. Sphingomyeline is then purified on an alumina column. It shows an N: P relation of 2:1, is free of carbohydrates and ester linkages and can be obtained in a yield of 87%.