Ein Verfahren zur quantitativen Isolierung reiner Sphingomyeline aus Menschen‐ und Rattenhirn | Zendy

Hausheer L. | Zendy; Pedersen W. | Zendy; Bernhard Karl | Zendy

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Ein Verfahren zur quantitativen Isolierung reiner Sphingomyeline aus Menschen‐ und Rattenhirn

Author(s) -

Hausheer L.,

Pedersen W.,

Bernhard Karl

Publication year - 1963

Publication title -

helvetica chimica acta

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.74

H-Index - 82

eISSN - 1522-2675

pISSN - 0018-019X

DOI - 10.1002/hlca.19630460220

Subject(s) - chemistry , sodium methoxide , chromatography , yield (engineering) , organic chemistry , methanol , materials science , metallurgy

A chromatographic method for the isolation of sphingomyelines from human and rat brain is reported. After isolation of the total lipids we first separate lecithine and sphingomyeline, on a silicagel column. Afterwards lecithine is split by transesterification with sodium methoxide. The cleavage products are separated from sphingomyeline on a silicagel column. Sphingomyeline is then purified on an alumina column. It shows an N: P relation of 2:1, is free of carbohydrates and ester linkages and can be obtained in a yield of 87%.

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