Premium
Die Fixierung von [ 14 C]‐Biotin an die Leberproteine des Hühnchens
Author(s) -
Gilgen A.,
Leuthardt F.
Publication year - 1962
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19620450614
Subject(s) - biotin , chemistry , avidin , incubation , biochemistry , enzyme
Abstract The fixation of carboxyl‐labelled biotin by chicken liver homogenate has been studied. Two independent mechanisms could be shown to exist: the first, a spontaneous and concentration dependent one, could be compared with the fixation of biotin to avidin. It is operative in extracts from both normal and biotin‐deficient animals. The second one, enzymic, requiring incubation in the presence of ATP, is inhibited by biotin analogues and can only be detected in extracts from biotin‐deficient animals. Part of the biotin‐proteins formed are enzymes. The proteins responsible for the spontaneous and for the enzymic fixation of biotin could partly be separated by ammonium sulfate fractionation.