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Quantitative Bestimmung von Nitroarginin in synthetischen Polypeptiden
Author(s) -
Gamper J. R.
Publication year - 1962
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19620450507
Subject(s) - chemistry , trifluoroacetic acid , anhydrous , dimethylformamide , chromatography , organic chemistry , solvent
A spectrochemical method for the assay of ω‐nitroarginine in synthetic peptides is described. It is based on the strong difference in absorption of the nitroarginyl residue in the two solvents dimethylformamide (DMF)/0,2 N HCl (1:1; v / v ) and anhydrous trifluoroacetic acid (TFA). The difference is greatest for nitroarginine and smallest for tryptophan and tyrosine, and most easily measured between the points 271.6 mμ (DMF/0,2 N HCl) and 267.6 mμ (TFA).