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Cerebrosid‐Gehalte verschiedener Bezirke alter menschlicher Gehirne Ein Beitrag zur quantitativen Isolierung reiner Cerebroside
Author(s) -
Bernhard K.,
Hany A.,
Hausheer L.,
Pedersen W.
Publication year - 1962
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19620450428
Subject(s) - chemistry , cerebroside , chromatography , elution , column chromatography , chloroform , biochemistry
An approximately quantitative method for the isolation of pure brain cerebrosides is reported. Crude cerebrosides are obtained out of total lipids by chromatography on an alumina column, pure cerebrosides are subsequently eluted with chloroform‐methanol from a silicagel column. Those are free of phosphatides, sphingomyelines, gangliosides and sulfatides.