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Untersuchung über die Biosynthese der Pterine bei Drosophila melanogaster
Author(s) -
BrennerHolzach O.,
Leuthardt F.
Publication year - 1961
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19610440606
Subject(s) - chemistry , glycine , drosophila melanogaster , ring (chemistry) , hydrolysis , fructose , acid hydrolysis , stereochemistry , biochemistry , amino acid , organic chemistry , gene
1. Larvae of the wild type of Drosophila melanogaster and of two mutants («trosy»)and («sepia») were fed with glycine‐ [2‐ 14 C], formate‐ [ 14 C] glucose‐ [U‐ 14 C], glucose‐(l‐ 14 C), glucose‐(6‐ 14 C), fructose ‐[U‐ 14 C] and DL ‐lactate‐ [1‐ 14 C]. After the hatching of the flies isoxanthopterine, drosopterine, sepiapterine and uric acid were isolated by paper chromatography and their specific activities were determined. The carbon atoms 8 and 9 of the pyrazine ring were isolated as oxalic acid after degradation of the pterine with chlorine followed by acid hydrolysis (method of W IELAND & T ARTTER ). The pyrimidine ring is not attacked by this process. This procedure was tested by the degradation of the synthetic isoxanthopterines‐ [8‐ 14 C] and ‐[5 14 C].