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Etude de structures peptidiques à l'aide de phénylisothiocyanate III. Sur la formation des phénylthiohydantoïnes‐[ 35 S] et sur leur chromatographie sur papier
Author(s) -
Cherbuliez Emile,
Sussmann A. R.,
Rabinowitz J.
Publication year - 1961
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19610440142
Subject(s) - chemistry , pentapeptide repeat , dimethylformamide , stereochemistry , peptide , biochemistry , solvent
By the analysis of di‐, tri‐peptides and a pentapeptide of known constitution the authors show that using an N,N‐dimethylformamide solution of [ 35 S]‐phenylisothiocyanate with a spectific activity of 10 to 40 mc/mmole the sequence of amino acids can be determined starting with 0,005 micromole of peptide.