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Die Bestimmung von Sulfonamiden in biologischem Material Vergleichende Untersuchungen der kolorimetrischen und der radiochemischen Methode an 35 S‐Sulfaphenazol
Author(s) -
Padowet W.,
Schmid K.,
Druey J.
Publication year - 1961
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19610440112
Subject(s) - chemistry , chromatography , calibration curve , detection limit
A known colorimetric method for the determination of sulfonamides in biological media has been modified and checked by means of radio‐assay using a 35 S labelled sulfonamide. Attention is drawn to the loss of sulfonamides during precipitation of proteins. It is shown that an accurate evaluation of results is only possible by using a calibration curve plotted on the basis of relevant recovery experiments.