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Etude de structures peptidiques à l'aide du phénylisothiocyanate II. Sur l'emploi de la microchromatographie d'adsorption sur couches minces des phénylthiohydantoïnes
Author(s) -
Cherbuliez Emile,
Baehler Br.,
Rabinowitz J.
Publication year - 1960
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19600430651
Subject(s) - chemistry , pyridine , heptane , silica gel , starch , solvent , adsorption , polymer chemistry , stereochemistry , chromatography , medicinal chemistry , organic chemistry
The microchromatography of phenylthiohydantoins on thin layers of silica (solvent: heptane, pyridine, ethyl acetate 5:3:2 vol.; revelation by starch‐iodineazide) is described. This method is 10 to 20 times more sensitive than that of classic paper chromatography. Good results are obtained in the E DMAN degradation of 0,01 μmole of di‐ or tri‐peptides.