Premium
Die enzymatische Dehydrierung der Stearinsäure zu Ölsäure
Author(s) -
Bernhard Karl,
Von BülowKöster J.,
Wagner H.
Publication year - 1959
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19590420112
Subject(s) - chemistry , stearic acid , oleic acid , fraction (chemistry) , enzyme , chromatography , microsome , biochemistry , fractionation , organic chemistry
We tried to prove the conversion of stearic acid into oleic acid by liver extracts. The method described by L E B RETON and co‐workers results in enzyme preparations from rat liver which decolorize methylenblue very rapidly but do not dehydrogenate stearic to oleic acid. After fractionation of liver cell particles in sucrose solution we were able to prove the existence of a dehydrogenase in the supernatant containing microsomes and cytoplasma, which converts 14 C stearic acid into oleic acid. The mytochondrial fraction showed no activity.