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Chemische und biologische Untersuchungen über ein Hämosensitin aus Mycobacterium tuberculosis
Author(s) -
Sorkin E.,
Boyden S. V.,
Rhodes J. M.
Publication year - 1956
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19560390628
Subject(s) - chemistry , hemagglutination , electrophoresis , polysaccharide , mycobacterium tuberculosis , agglutination (biology) , specific rotation , chromatography , microbiology and biotechnology , stereochemistry , biochemistry , antigen , tuberculosis , medicine , pathology , biology , genetics
Chemical studies have been carried out on polysaccharide fractions from culture filtrates of M. tubercdosis which sensitize sheep red cells to haemagglutination by antituberculous sera (Middlebrook‐ Dubos test). The active substance has been called α‐haemosensitin. The most highly purified preparations have an optical rotation of [α] 20 D+72° (water) and sensitize red cells to agglutination at a concentration of 0.001 mg/ml. The purity of the products was tested by chemical analysis, paper electrophoresis, boundary electrophoresis and serological means. The electrophoretic data suggest that even the most active preparations are not homogenous, probably because of the presence of more than one structurally closely related type of polysaccharide molecules.