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Über den Mechanismus der Glutaminsynthese
Author(s) -
Staehelin M.,
Leuthardt F.
Publication year - 1955
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.19550380121
Subject(s) - chemistry , adenylate kinase , enzyme , glutamine , phosphorylation , stereochemistry , biochemistry , medicinal chemistry , amino acid
1. By incubating the glutamine synthesizing enzyme with AD 32 P and ATP the latter became radioactive by exchange with AD 32 P. Our enzyme preparation still contained some myokinase which it was impossible to remove. Using suitable inhibitors (mercurochrome, 0,1‐m. CaCl 2 ) we show that the exchange is only partly due to myokinase. There exists a second reaction, catalysed by glutamine synthese, which may be explained by phosphorylation of the enzyme, thus:.